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RNasin|RNase Inhibitors|RNase
Inhibition|RNA Protection
Catalog # |
Pack size(40U/ul) |
Price(USD) |
ZP00801-S |
5000U(125ul) |
$90.00 |
|
ZP00801--M |
20000U(500ul) |
$280.00 |
ZP00801--L |
40000U(1ml) |
$500.00 |
Description
RNasin(RNase Inhibitor) is a recombinant mammalian RNase
inhibitor that is expressed as a soluble protein in E. coli.
with a molecular weight 51 kDa. It is a noncompetitive
inhibitor of RNases A, B and C, human placental RNase and
angiogenin. RNasin, free of RNase or Nickase, can maintain
its activity at pH from 5 to 8, with the highest activity at
pH 7.8. The concentration of RNasin is 20~40 units/µl.
RNasin has been tested in RT-PCR and is compatible with
enzymes such as AMV, M-MLV Reverse Transcriptases or Taq DNA
polymerases. It has also been tested and found to be
compatible with quantitative, real-time RTPCR reactions in a
TaqMan Assay.
Features:
l
Higher stability-up to 15 minutes at 70 °C
l
Activity of eukaryotic RNaseA, RNaseB, RNaseC and human
placenta RNase
l
Wide pH activity ranges. (1 mM DTT in the buffer system is
needed.)
Application:
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RT-qPCR、multiplex
RT-qPCR
l
RNA isolation or RNA store
l
In vitro transcription or any situation where RNase
contamination is a concern.
RNasin
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