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Custom Gene Synthesis
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Oligo Synthesis               


ShineGene has synthesized millions of high-quality oligonucleotides for researchers since it was founded in 2003. The oligos have been applied in almost all fields of molecular biology, and have become the top choice for researchers.ShineGene offer you an innovative line of custom oligonucleotides for your research. As always, every custom DNA oligo is deprotected and desalted – quality is always guaranteed!
To meet the increasing market demand, we have introduced the most advanced high-throughput synthesizers, which provide computer-controlled oligo synthesis, cleavage, and deprotection in a parallel-processing format. Our oligos are synthesized with a typical coupling efficiency of 99% and purified by OPC free of charge. PAGE and HPLC purification are also available with additional charge.
 
Note:Estimate 1 OD=5 nmols=33 µg,for a 20 base oligo
         Shipping is extra.Freight is 35.00
 
Scale Purification Type Yield(OD) 
40nmol OPC 2-10 OD
Page 1-5 OD
HPLC 1~2 OD
200nmol OPC 5-20 OD
Page 2-8OD
HPLC 1-5OD
1000nmol OPC 40-80 OD
Page 20-40 OD
HPLC 1-40 OD

Oligo Synthesis Order Form  
Standard Oligos

Yield Price(US$/base) Working days
2 OD 0.2 2~3days
5 OD 0.23
10 OD 0.4
30 OD 0.9
60 OD 1.65
100 OD 2.7
The price is only for oligos shorter than 60 mer.OPC purified.

Probes for Quantitative, Real-time PCR
 

Taqman probes 2 OD (US$) 5 OD (US$) 10 OD (US$)
Taqman probes with a Black Hole Quencher
5'FAM and 3'BHQ-1 161 225 322
5' HEX and 3' BHQ-1 193 257 386
5' Vic and 3' BHQ-1 300 400 550
5' TET and 3' BHQ-1 193 257 386
5' Cy3 and 3' BHQ-2 185 249 370
5' Cy5 and 3' BHQ-2 242 306 484
5' NED and 3' BHQ-2 300 400 550
5' Joe and 3' BHQ-1 242 306 484
5' TAMRA and 3' BHQ-2 161 225 322
5’-CAL Fluor Red 610 and 3’ BHQ-2 322 386 644
5’-Quasar 670 and 3’ BHQ-2 322 386 644
5’-Quasar 570 and 3’ BHQ-2 403 467 806
Taqman probes with a TAMRA Quencher
5' FAM and 3' TAMRA 145 209 290
5' HEX and 3' TAMRA 161 225 322
5' TET and 3' TAMRA 161 225 322
 
Turn-around time is about 5-7 working days

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Molecular Beacon 2 OD (US$) 5 OD (US$) 10 OD (US$)
5'-FAM and 3' Dabcyl 193 257 386
5'-HEX and 3' Dabcyl 241 305 482
5'-TET and 3' Dabcyl 241 305 482
5'-CY3 and 3' Dabcyl 300 364 600
5'-CY5 and 3' Dabcyl 300 364 600
5'-TAMRA and 3' Dabcyl 193 257 386
 
Turn-around time is about 5-7 working days

 

TaqMan MGB PROBE 2 OD (US$) 5 OD (US$)
5'-FAM and 3' MGB 564 1015
5'-VIC and 3' MGB 645 1161
5'-NED and 3' MGB 645 1161
 
Turn-around time is about 5-7 working days



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Fluorescent Oligos    
       
Fluorophores 2 OD (US$) 5 OD (US$) 10 OD (US$)
5'-FAM 64 72 128
5'-HEX 104 128 208
5'-TET 104 128 208
5'-CY3 208 256 416
5'-CY5 208 256 416
5'-TAMRA 104 128 208
3'-Fluorescein 64 72 128
3'-TAMRA 104 128 208
3'-Cy3 208 256 416
3'-Cy5 208 256 416
3'-Dabcyl 104 128 208
3'-ROX 128 144 256
3'-BHQ-1 128 144 256
3'-BHQ-2 128 144 256
3'-BHQ-3 128 144 256
For long oligos (60~100 mer) multiply 1.4 based on the above prices
Turn-around time is about 3-4 working days.
Other labelings are available upon request.
Oligo Purification    
  OPC PAGE (US$/oligo) HPLC (US$/oligo)
2 OD Free 20 20
5 OD Free 30 30
10 OD Free 60 60
30 OD Free 150 150
50 OD Free 180 180
100 OD Free 240 240
OPC purification is only for < 60 mer standard oligos and S-oligos.
Long oligos ( 60 mer) should be PAGE purified.
Modified oligos should be PAGE or HPLC purified.


Oligo Synthesis Order Form    


If ordering by P.O., please send an E-mail to syn@synthesisgene.com or containing the P.O.#, shipping address and billing address. Please provide a phone number for delivery purposes.

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Floor 2,Building A,328#, Wuhe Road,Minhang District,Shanghai,201109,China
Tel:0086-21-54460832    Fax:0086-21-54460831-13    E-mail:syn@synthesisgene.com