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Real Time Quantitative PCR Service(qPCR Service)

Real-time quantitative PCR remains one of the most sensitive and quantitative tools for gene expression used today.
Quantitative PCR(qPCR) is a powerful, highly sensitive technique that can be used to quantitate gene expression, determine gene copy number, detect SNPs, and detect DNA from viral and bacterial microorganisms. ShineGene support both SybrGreen and Taqman reaction chemistries. With Taqman chemistry, a labeled probe is included in the reaction to enhance the specificity and sensitivity of target sequence detection. With SyberGreen chemistry, amplified product is detected by its interaction with the SyberGreen fluorescent dye, which selectively binds to double-stranded DNA templates. We also offer primer and probe design services. We manage all aspects of the project from assay design to data analysis. Sample preparation (Nucleic acid isolation, whole genome amplification, etc.) is also available. You can get full service from ShineGene.

Quantitative PCR can be applied in several key areas including:
1.Gene Expression Analysis
2.SNP Polymorphism Analysis
3.Quantification of miRNA

4.Drug Response Analysis
5.Cell Bank Copy Number Analysis
6.Mutation Analysis
7.Residual DNA Analysis

1.Chou, Q., Russell, M., Birch, D., Raymond, J. & Bloch, W. (1992) Prevention of pre-PCR misprim­ing and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res.20:1717?C1723.
2.Roux, K. H. (1995) Optimization and troubleshooting in PCR. PCR Methods Appl. 4:5185?C5194.

3.Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(t)) Method.Methods 2001, 25:402-408.

4.L D Ke, Z Chen .A reliability test of standard-based quantitative PCR: exogenous vs endogenous standards Mol Cell Probes. 2000 Apr;14(2):127-35.
5.Weihong Liu and David A. Saint Validation of a quantitative method for real time PCR kinetics Biochemical and Biophysical Research Communications 294 (2002) 347?C353
6.Heid, C.A., et al. Real time quantitative PCR. Genome Research 6(10), 986-94, 2002.
7.Pusterla, N., et al. Quantitative real-time PCR for detection of members of the Ehrlichia phagocytophila genogroup in host animals and Ixodes ricinus ticks. J. Clin. Microbiol. 37(5), 1329-31, 1999.
8.Saito T., et al. Quantitative DNA analysis of low-level hepatitis B viremia in two patients with serologically negative chronic hepatitis B. J. Med. Virol. 58(4), 325-31, 1999.
9.Tyagi, S., and F. R. Kramer. Molecular beacons probes that fluoresce upon hybridization. Nature Biotechnology 14(3), 303-8, 1996.
10.Tyagi S., et al. Wavelength-shifting molecular beacons. Nature Biotechnology 18(11), 1191-96, 2000.
11.Park S., et al. Rapid identification of Candida Dubliniensis using a species-specific molecular beacons.J. Clin. Microbiol. 38(8), 2829-36, 2000.

Price List
Full service (from sample to data acquisition)You can get full service from ShineGene.E-mail:master@shinegene.org.cn

RNA Isolation from Sample 


cDNA Synthesis




Taqman Probe


Sybr Green I

be free

The turn around time from sample to data is about 30 working days.

Sample Submission Requirements
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Floor 2,Building A,328#, Wuhe Road,Minhang District,Shanghai,201109,China
Tel:0086-21-54460832    Fax:0086-21-54460831-13    E-mail:master@shinegene.org.cn