Custom siRNA Construction Services Cat.No.:SG00105 Gene silencing of target
mRNA by RNA interference (RNAi) has dramatically
enhanced the arsenal of genetic tools that can be used
to study gene function. RNAi is a process used by plants
and invertebrates to specifically inactivate gene
function. It has been shown that double-stranded RNA (dsRNA)
complementary to particular messenger RNA (mRNA) can
specifically inactivate gene function through the RNAi
pathway in mammalian systems as well. Small interfering
RNAs (siRNAs) are generated in vivo from long precursor
molecules through the action of a specific RNAase III
endonuclease called Dicer. Dicer initiates the cleavage
of the precursor into ~22 nucleotide (nt)
double-stranded duplexes with two nt 3'-overhangs and 5'
phosphate termini termed short interfering RNAs (siRNAs).
The siRNA is subsequently incorporated into the RNA
induced silencing complex (RISC), a protein/RNA complex.
The siRNA allows specific recognition of the mRNA target
and the endonuclease function of the RISC results in
cleavage of mRNA and thus loss of gene expression.
We will design, construct, and package siRNA-expressing
vector constructs targeting your gene of interest. You
only provide the GenBank (or other public database)
accession number to a gene sequence and choose one of
our siRNA cloning and expression vectors. We can provide
the following service:
1.Select siRNA sequences based on parameters that, in our
experience, are most likely to select effective siRNA.
2.Synthesize and clone template sequences for the siRNA into
a vector of your choice to produce siRNA-expressing
vector constructs.
3.Sequence the constructs to confirm correct cloning.
