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Subcloning Service

Subcloning is a technique used to move a particular gene of interest from a original vector to a destination vector in order to further study its functionality,especially in protein expression.
ShineGene’s subcloning procedure also provides gene manipulation including tag fusion, cleavage site insertion. If our clients do not have a gene in hand, we can clone the gene based on the accession number our client provided.
Human cDNA Clone

To order, please provide:
1. Desired target gene sequence, cloning vector and cloning sites.
2. The template and its sequence information,Chromas sequence.
3. If the starting material is plasmid, a 1-2 µg template plasmid droped on filter paper.
A sufficient amount of culture if starting material is bacterial culture.
4. Maps, antibiotic resistance, and cloning sites of destination vectors.

Email:master@shinegene.org.cn or shinegene@vip.163.com     Subcloning Service Order Form

ShineGene will
1. Perform PCR cloning, subcloning or mutagenesis to get the desired target construct.
2. Deliver about 1-4ug of plasmid with sequence chromatograms(electronic) covering your gene. All constructs are confirmed by DNA sequencing.
3.The turnaround is about 14 working days depending on the complexity of the subcloning project.

Subcloning/PCR cloning Price:
For gene less than 1kb, Eur120 for Subcloning/PCR cloning
The above list price is only for genes which is less than 1 kb. For longer gene, there is Eur50 additional cost per kb for screening and sequencing.

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DNA Sequencing
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Lab Consumables

gene synthesis


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Floor 2,Building A,328#, Wuhe Road,Minhang District,Shanghai,201109,China
Tel:0086-21-54460832    Fax:0086-21-54460831-13    E-mail:master@shinegene.org.cn