Site-directed mutagenesis is a
molecular biology technique in which a mutation is
created at a defined site in a DNA molecule, usually a
circular molecule known as a plasmid. In general,
site-directed mutagenesis requires that the wild type
gene sequence be known.
This technique is also known as site-specific
mutagenesis or oligonucleotide-directed mutagenesis.
Site-directed mutagenesis has been widely used in the
study of protein functions.
ShineGene's mutation procedure also provides gene
manipulation include point mutations, deletions, and
insertions. They can be customized to fit any scientific
order, please provide:
1. Desired target gene sequence, cloning vector and cloning
2. The template and its sequence information,Chromas
3. If the starting material is plasmid, a 1-2 µg template
plasmid droped on filter paper.
A sufficient amount of culture if starting material is
4. Maps, antibiotic resistance, and cloning sites of
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1. Perform PCR cloning, subcloning or mutagenesis to get the
desired target construct.
2. Deliver about 1-4ug of plasmid with sequence
chromatograms(electronic) covering your mutation. All constructs
are confirmed by DNA sequencing.
3.The turnaround is about 14 working days depending on the
complexity of the mutagenesis project.
gene less than 1kb,
gene more than 1kb,
Pfu DNA Polymerase
Taq DNA Polymerase