Send IM to Anne
Send IM to Peter
 
  English | 中文
 
 
 
1
Taq Polymerase for qPCR
HotStart Taq Polymerase
Pfu DNA Polymerase
Kod DNA Polymerase
dNTPs
Reverse Transcriptase
MiniRNA Prep
First Strand cDNA Synthesis Kit
Real-Time qPCR Kit
One Step qPCR Kit
DNA Marker
Lab Consumables
Lab Equipments
SARS-CoV-2 Probes
SARS-CoV-2
SARS-CoV-2 IgM/IgG Antibody Rapid Test
Free 2019-nCoV Plasmids
Spin Column for  DNA/RNA
1

LinkedIn Google

SARS-CoV-2(2019-nCOV) Coronavirus and Influenza A/B Virus Multiplex RT-qPCR Detection Kits

Catalog #

Pack size

Price($)

COV00102

50tests

1200.00

COV00103

100tests

2160.00

This kit is used for in vitro diagnostics for 2019-Novel Coronavirus (2019-nCoV) infection including suspected case, suspected aggregative diseases and other scenarios. By testing the suspected person’s respiratory specimen, serum or blood, the kit can tell if the specimens contain any virus RNA, so it can be used for clinical screening of suspected cases.
       Select the 2019-nCoV N gene and E gene as the target amplifying area, design specific primer and fluorescent probes, use FAM label, detect if the specimen contains any 2019 nCoV RNA. Select the 2019-nCoV ORF1ab gene, design specific primer and florescent probes, use ROX label, detect if the specimen contains any 2019-nCoV RNA. Select influenza A/B specific sequence, design specific primer and fluorescent probes, use Cy5 label, detect if the specimen contains any influenza A/B virus RNA. Select other species’ sequence (none-human and none-targeted sequence) as internal primer/probe, label with VIC, monitor the whole process.
        The kit also contains UNG enzyme anti-contamination system. Its theory is it selectively hydrolyzes Uracil glycoside bond in the single or double chain of DNA that contains dU. This leads to a DNA chain with nucleotide deletion which will further hydrolyzed in alkaline media under high temperature and thus avoid contamination by PCR amplification.

                                                                        4
Fluorescent Signals and Target

 

Fluorescent signal

Target

1

FAM

2019-nCoV N gene and E gene

2

ROX

2019-nCoV ORF1ab gene

3

Cy5

Influenza A/B specific sequence

4

VIC

MS2 internal standard (RNA Phage pseudo virus)

 Key Features:  
1.High sensitivity 500 copy/ml
2.Very specific, can detect 2019-nCoV E gene, N gene and ORF1ab different zone sequence at the same time in one tube.
3.Can simultaneously identify and distinguish influenza viruses to avoid misdiagnosis.
4.One-step RT-PCR, strong amplification signal, high stability.
Storage condition:  -20°C.
Equipment Requirements: Fluorescent PCR amplifier; Fluorescent PCR amplifier with 4 colors or multi-color fluorescent PCR amplifier  such as  ABI Prism®7500, Bio-rad CFX96 etc.
Set up reaction mixture:

Component

VolumeμL

RT-mix

Nx 9

Enzyme mix

Nx 1.5

2019-nCoV primer probe

Nx 1

Influenza A/B primer probe

Nx 1

Total volume

Nx 12.5

Set up cycle program::
 

Step

Temp

Time

Cycles

1

UNG Incubation

25°C

2min

1

2

RT incubation

50

10min

1

3

Enzyme activation

95

2min

1

4

Denature

95

5sec

45

Annealing, extension, detction*

60

35sec

*Fluorescent test in extension:
Channel for 2019-nCoV E gene and N gene is FAM; Channel for 2019-nCoV ORF1ab gene is ROX;
Channel for influenza A/B is Cy5;
Channel for internal standard is VIC;

Data Analysis:

Ct Value*

Result

Recommendations

CtFAM≤36.5 and CtROX≤38

2019-nCoV positive(+)

Active treatment

CtFAMROX≥39 or no valueand CtVIC)<40

2019-nCoV negative(-)

More Rest

CtFAMROX≥39 or no valueand CtVIC)<40,but Ct(Cy5) ≤36.5

2019-nCoV negative(-)

Influenza A/B positive(+)

Active treatment

CtFAM≤36.5 and CtROX≤38,and Ct(Cy5) ≤36.5

2019-nCoV negative(+)

Influenza A/B positive(+)

Active treatment

 only CtFAM≤36.5 or CtROX≤38

 Inconclusive Result

Repeat extraction and double confirm. After getting CtFAM≤36.5 or CtROX≤38, the result is positive

  

36.5CtFAM)<39 or 38CtROX)<39

  

Inconclusive Result

Increase specimen concentration and double confirm. If CtFAM)<39 and CtROX)<39, it means 2019-nCoV result is positive; other results need be analyzed by other methods.

 CtFAMROXCY5≥39 or no valueand CtVIC≥40 or no value

 Invalid Result

Indicates that there might be issues with experiment itself, reagent, need check before repeat the experiment

SARS-CoV-2(2019-nCOV) Coronavirus and Influenza A/B Virus Multiplex RT-qPCR Detection Kits
  Handbook of COVID-19 Prevention and Treatment

If you want to get bulk order price,please contact us.E-mail:master@shinegene.org.cn or shinegene@vip.163.com

LinkedIn Google

Related Link
SARS-CoV-2 Primer and Probe
Oligo Synthesis
Taqman probe
Peptide Synthesis
Chromas
Make Antibody
Lab Consumables
Gene Synthesis
dNTP


 

 

Copyright 2003-2020 Shanghai ShineGene Molecular Biotech,Inc. All Rights Reserved.
Floor 2,Gate 6,289#,Minqiang Road,Songjiang District,Shanghai 201612,China
Tel:0086-21-54460832    Fax:0086-21-54460831-13    E-mail:master@shinegene.org.cn