Description: DNA
polymerase from Thermococcus kodakaraensis KOD is one of
the most efficient thermostable PCR enzymes exhibiting
higher accuracy and elongation velocity than any other
commercially available DNA polymerase.The enzyme
catalyzes the template-dependent polymerization of
nucleotides into duplex DNA in the 5'---->3'
direction. The KOD DNA Polymerase also exhibits 3'--->5'exonuclease (proofreading) activity, that
enables the polymerase to correct nucleotide
incorporation errors. It has no 5'--->3'
exonuclease activity.
Concentration:5U/ul
Source: KOD
DNA Polymerase (native) - Thermococcus kodakaraensis
cells.KOD DNA Polymerase (recombinant) - E.coli cells
with a cloned pol gene from Thermococcus kodakaraensis. 10X Reaction Buffer with
MgCl2:
1
ml 10X Buffer : 1.2 M Tris-HCl, 100 mM KCl, 60 mM (NH4)2SO4,
25 mM
MgCl2,
1% Triton X-100, 0.01% BSA, pH8.0 .
Features:
High
fidelity than other normal DNA polymerase
Higher
processivity.
Higher
yield in short time
Applications:
All
PCR* amplification which demands high fidelity.
Primer
extension.
High
fidelity PCR for cloning into blunt-ended vectors.
Site-directed
mutagenesis.
Unit
Definition: One
unit is defined as the amount of enzyme that will
catalyze the incorporation of 10 nmol dNTP into acid
insoluble form in 30 minutes at 75°C in a reaction
containing 20 mM Tris-HCl (pH7.5 at 25°C), 8 mM MgCl2,
0.5 mM DTT, 50ug/ml
BSA,150
uM
each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled
and [3H]-dTTP) and 150
ug/ml activated calf
thymus DNA.
Quality
Control Tests:
PCR (activity), SDS-PAGE (purity), endonuclease/nickase.
Storage: 5U/ul in 50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM
EDTA, 50% glycerol, 0.1% Nonidet P-40, 0.1% Tween-20,
pH8.0
